Important: Participation in our Fiji/ImageJ beginner course or the Biovoxxel ImageJ/Fiji course, or detailed knowledge of Fiji/first experiences with macros or other programming languages is required. Topics: detailed lecture about ImageJ macro language, step-by-step programming tutorial, open to discuss and write own macros Image processing with Fiji/ImageJ – Advanced course (macro programming) Topics: overview and short introductions to advanced Fiji plugins, open source software (Ilastik/Cellprofiler) as well as purchased software available at Münster University like Imaris (3D Analysis) and Huygens Professional (deconvolution & general image restoration) Image processing with advanced image analysis software & tools Microscopy – what’s at hand at Münster University?ĭifferent microscopy systems, services (OMERO/analysis server), image analysis, provided software, examples of methods from different groups Research data management for microscopy, OMERO, data storage, analysis server Overview about the services of the Imaging Network and the available microscopy systems (database & data storage, analysis server, image analysis and more) Münster Imaging Network – Services & Microscopy Systems Wild field systems, point spread function, numerical aperture of an objective, resolution (raylight, FWHM, Abbe diffraction limit), different kinds of fluorophores Research data management for microscopy dataĬonfocal laser scanning microscopes, beampath, detectors (PMT, HyD, GaAsp), signal to noise ratio, tips for live cell imaging, sample preparation STORM, PALM, DNA-PAINT), single particle trackingĭeconvolution (Huygens), Airyscan (Zeiss), Lightning (Leica), deep learning approaches (N2V / CARE) Single molecule localization microscopy (SMLM, e.g. Previous topics and dates Date, time and place.The log-in information below is valid for all upcoming events of this seminar series. Our meetings are currently held online via Zoom. Speaker: Thomas Zobel, Coordinator Microscopy – Imaging Network Everyone working at Münster University, and especially master and doctoral students, are welcome to participate! Topics and dates The techniques covered include fluorescence recovery after photobleaching (FRAP), fluorescence resonance energy transfer (FRET) and fluorescence-lifetime imaging microscopy (FLIM), super-resolution methods such as stimulated emission depletion (STED) and single molecule localization microscopy (SMLM), and single particle tracking. We provide an introduction into different instruments and methods for fluorescence microscopy – starting with the preparation of samples as well as basic functions and instrument settings up to characteristics in the technical design of special microscopes.
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